منابع مشابه
Chlamydomonas Flagella
Methods were developed for the isolation of Chlamydomonas flagella and for their fractionation into membrane, mastigoneme, "matrix," and axoneme components. Each component was studied by electron microscopy and acrylamide gel electrophoresis. Purified membranes retained their tripartite ultrastructure and were shown to contain one high molecular weight protein band on electrophoresis in sodium ...
متن کاملBiochemistry of Chlamydomonas flagella.
The flagella of Chlamydomonas can be detached from the cell bodies by calcium ions. These flagella round up and some of the axoneme protein goes into solution when they are resuspended in a buffer containing magnesium as used by Gibbons (1963) and Watson & Hynes (1966) for preserving Tetrahymena cilia. Flagella showing a normal transverse section and good membrane preservation may be obtained b...
متن کاملParalyzed Flagella Mutants of Chlamydomonas reinhardtii
Five paralyzed flagella mutants of Chlamydomonas reinhardtii have been isolated and characterized ultrastructurally as specifically defective for either the outer row or inner row of arms on the axonemal doublet microtubules. Four mutants falling in two genetic loci (pf-13, pf-ItA, pf-22, and pf-22A) show quantitative deficiencies for outer arms. One mutant for a third locus, pf-23, is deficien...
متن کاملRadial spoke proteins of Chlamydomonas flagella.
The radial spoke is a ubiquitous component of '9+2' cilia and flagella, and plays an essential role in the control of dynein arm activity by relaying signals from the central pair of microtubules to the arms. The Chlamydomonas reinhardtii radial spoke contains at least 23 proteins, only 8 of which have been characterized at the molecular level. Here, we use mass spectrometry to identify 10 addi...
متن کاملShort-Flagella Mutants of Chlamydomonas reinhardtii.
Six short-flagella mutants were isolated by screening clones of mutagenized Chlamydomonas for slow swimmers. The six mutants identify three unlinked Mendelian genes, with three mutations in gene shf-1, two in shf-2 and one in shf-3. shf-1 and shf-2 have been mapped to chromosomes VI and I, respectively. Two of the shf-1 mutations have temperature-sensitive flagellar-assembly phenotypes, and one...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1972
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.54.3.540